Enzymes and Inhibitors
Bachem H-Ala-AMC
H-Ala-AMC, 77471-41-1, C13H14N2O3, Mr 246.27, 1g. H-Ala-MCA (free base). Special fluorogenic substrate for aminopeptidase M (microsomal alanyl aminopeptidase, membrane aminopeptidase, APN). Furthermore, the major aminopeptidase from human skeletal muscle showed highest activity with this substrate.
New England Biolabs, Inc. I-SceI – 2500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. XbaI – 15000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. BamHI – 10000 units
A restriction endonuclease that recognizes the sequence G^GATC_C.
New England Biolabs, Inc. MspI – 25000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. BstXI – 5000 units
A restriction endonuclease that recognizes the sequence CCAN_NNNN^NTGG.
New England Biolabs, Inc. MboII – 1500 units
A restriction endonuclease that recognizes the sequence GAAGANNNNNNN_N^.
New England Biolabs, Inc. AvaII – 10000 units
A restriction endonuclease that recognizes the sequence G^GWC_C.
New England Biolabs, Inc. HpaII – 10000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. SphI – 2500 units
A restriction endonuclease that recognizes the sequence G_CATG^C.
New England Biolabs, Inc. BspHI – 2500 units
A restriction endonuclease that recognizes the sequence T^CATG_A.
Cell Signaling Technology PathScanR Phospho-c-Abl panTyr Sandwich ELISA Kit 10 Kit
PathScanR Phospho-c-Abl panTyr Sandwich ELISA Kit 10 Kit
Ubpbio SUC-LLVY-AMC, 25MG
Fluorescent Peptides/Protein Substrates – SUC-LLVY-AMC (UBPBio catalog# G1100, G1101) – Suc-LLVY-AMC is a fluorogenic substrate for the chymotrypsin – like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Ubpbio BOC-LRR-AMC, 5MG
Fluorescent Peptides/Protein Substrates – BOC-LRR-AMC (UBPBio catalog# G3100, G3101) – Boc-LRR-AMC is a fluorogenic substrate for the trypsin – like activity of the 20S proteasome. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
New England Biolabs, Inc. T4 Phage beta-glucosyltransferase – 2500 units
T4 Phage -glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine.